abstract：:A novel, inexpensive apparatus (locometer) was designed to measure behavioral activity of rats including gross locomotor activity, rearing, and structural characteristics of movement patterns (i.e. fractal dimension). Measurements were done in 3 dimensions at a resolution of approximately 1 cm. To test the sensitivity...

journal_title：Journal of neuroscience methods

authors： Brodkin J,Nash JF

更新日期：1995-04-01 00:00:00

abstract：:The present study describes the use of nose-poke habituation as a memory task and demonstrates that it is sensitive to hypo- and hypermnestic pharmacological treatments administered post-trial. Habituation of nose-poke behavior of rats was defined as a reduction in number of nose-pokes compared to baseline. It was mea...

journal_title：Journal of neuroscience methods

authors： Voits M,Fink H,Gerhardt P,Huston JP

更新日期：1995-03-01 00:00:00

abstract：:The measurement of amino acid neurotransmitters by high-performance liquid chromatography (HPLC) has emerged as a reliable and sensitive method. This paper describes a method which employs electrochemical (EC) detection of amino acid derivatives formed by a reaction with o-phthalaldehyde (OPA) in the presence of sulph...

journal_title：Journal of neuroscience methods

authors： Rowley HL,Martin KF,Marsden CA

更新日期：1995-03-01 00:00:00

abstract：:The EPC-9 patch-clamp amplifier is a digitally controlled analog device for recording currents in membrane patches and in small cells. It has neither front-panel controls nor internal trim adjustments; instead all gain, range-changing, transient cancellation, calibration and other functions are computer controlled. No...

journal_title：Journal of neuroscience methods

authors： SIGWorth FJ

更新日期：1995-02-01 00:00:00

abstract：:Sensory and motor pathways in the central nervous system (CNS) of macaque monkeys were visualized by anterograde or retrograde axonal transport of wheatgerm agglutinin-horseradish peroxidase (WGA-HRP) reacted with the chromagen tetramethylbenzidine (TMB), or by the use of anterograde degeneration after specific ablati...

journal_title：Journal of neuroscience methods

authors： Milroy AM,Ralston DD

更新日期：1995-02-01 00:00:00

abstract：:There are many reasons for wishing to determine the rate of uptake of a drug from blood into brain parenchyma. However, when faced with doing so for the first time, choosing a method can be a formidable task. There are at least 7 methods from which to choose: indicator dilution, brain uptake index, microdialysis, exte...

journal_title：Journal of neuroscience methods

authors： Bonate PL

更新日期：1995-01-01 00:00:00

abstract：:Three-dimensional (3D) reaggregate tissue culture provides a means of quantitatively assessing neuronal cell survival under a variety of experimental conditions. A method is presented for estimation of, and comparison between, the total numbers of cells of a given neurochemical type within individual experimental flas...

journal_title：Journal of neuroscience methods

authors： Vidal L,Heller B,Won L,Heller A

更新日期：1995-01-01 00:00:00

abstract：:The effects of the microwave antigen-retrieval method were investigated for five antibodies commonly used in neurosciences, i.e., Parvalbumin, Calbindin D28-K, MAP-2, MAP-5 and SMI-32. Immunocytochemical stainings were performed on free-floating vibratome sections of tissue which had been stored in fixative for as lon...

journal_title：Journal of neuroscience methods

authors： Evers P,Uylings HB

更新日期：1994-12-01 00:00:00

abstract：:Antibody microprobes are devices which have been used to determine the release sites of several neuropeptides. The production of microprobes using the previous methodology is a time-consuming procedure requiring a high level of skill. The aim of the study was to investigate alternative methods for the production of mi...

journal_title：Journal of neuroscience methods

authors： Waterfall AH,Clarke RW,Bennett GW

更新日期：1994-11-01 00:00:00

abstract：:Functional magnetic resonance imaging (FMRI) can provide detailed images of human brain that reflect localized changes in cerebral blood flow and oxygenation induced by sensory, motor, or cognitive tasks. This review presents methods for gradient-recalled echo-planar functional magnetic resonance imaging (FMRI). Also ...

journal_title：Journal of neuroscience methods

authors： DeYoe EA,Bandettini P,Neitz J,Miller D,Winans P

更新日期：1994-10-01 00:00:00

abstract：:Transplantation of human fetal ventral mesencephalon (VM) to Parkinsonian patients has shown beneficial effects in several clinical trials. However, further improvements in the transplantation technique are needed. Delayed surgery, i.e., the in vitro maintenance of the tissue prior to transplantation would present sev...

journal_title：Journal of neuroscience methods

authors： Spenger C,Studer L,Evtouchenko L,Egli M,Burgunder JM,Markwalder R,Seiler RW

更新日期：1994-09-01 00:00:00

abstract：:A method for quantitative estimation of sensory neuron sensitivity to small sinusoidal stimuli in the presence of sizable background drift (in the stimulus or response) was developed. The performance of the method was tested by analyzing the responses of 17 muscle spindle primary (Ia) afferent neurons to concomitant s...

journal_title：Journal of neuroscience methods

authors： Hulliger M,Baumann TK

更新日期：1994-08-01 00:00:00

abstract：:We describe a much simplified high-performance liquid chromatography (HPLC) method for the measurement of caffeine in plasma and brain. A particularly attractive feature of this method is that a simple methanol/water (60:40) mobile phase can be used both for plasma and brain samples. In addition, the method is compati...

journal_title：Journal of neuroscience methods

authors： Carey RJ,DePalma G

更新日期：1994-07-01 00:00:00

abstract：:Senile plaques (SP) are the most characteristic neuropathologic lesions of Alzheimer's disease (AD) and studies of plaque cortical distribution, density, and morphology may lead to new information about the origin and pathogenesis of this disease. We have developed an automated computer image analysis program to detec...

journal_title：Journal of neuroscience methods

authors： Hibbard LS,Arnicar-Sulze TL,McKeel DW Jr,Burrell LD

更新日期：1994-06-01 00:00:00

abstract：:This paper describes a simple multichannel hook electrode specially adapted for simultaneous recording from several dorsal root filaments in the cat. The electrode consists of a 3-mm-thick PVC plate and accommodates 12 silver wire hooks placed in notches in the plate. It has a semicircular shape, and the notches are m...

journal_title：Journal of neuroscience methods

authors： Djupsjöbacka M,Johansson H,Bergenheim M,Sandström U

更新日期：1994-04-01 00:00:00

abstract：:The present study concerns an ELISA for glial fibrillary acidic protein (GFAP) in cerebrospinal fluid (CSF). The application of the method in CSF of children has previously been described in this journal. We have now adapted the technique to allow determination of the much higher GFAP concentrations found in normal an...

journal_title：Journal of neuroscience methods

authors： Rosengren LE,Wikkelsø C,Hagberg L

更新日期：1994-03-01 00:00:00

abstract：:The technique that we describe aims at evaluating the numerical density of cells in highly heterogeneous regions, e.g., nuclei, layers or columns of neurones. Rather than counting the number of neuronal sections ('profiles') in a reference frame, we evaluated the 'free area' which lies around each profile. The X and Y...

journal_title：Journal of neuroscience methods

authors： Duyckaerts C,Godefroy G,Hauw JJ

更新日期：1994-01-01 00:00:00

abstract：:A technique is described to measure the electrically evoked increase in intracellular calcium in cerebellar granule cells cultured on glass coverslips and preloaded with FURA-2. To minimize light scattering, the coverslip containing the granules was placed in the fluorimeter cuvette at a 30 degrees angle to the exciti...

journal_title：Journal of neuroscience methods

authors： Beani L,Tomasini C,Govoni BM,Bianchi C

更新日期：1994-01-01 00:00:00

abstract：:A biotinylated lectin from Ricinus communis (RCA-I) and avidin-biotin-horseradish peroxidase complex (ABC) were used to identify microglial cells in 3-microns-thick sections of formalin-fixed paraffin-embedded brains of adult mice required for quantitative cell kinetic studies. In 3-microns-thick sections of the mouse...

journal_title：Journal of neuroscience methods

authors： Hauke C,Korr H

更新日期：1993-12-01 00:00:00

abstract：:A method for selecting a usable carbon-fiber electrode using the equivalent resistance and capacitance is presented. This method uses an instrument with a PC-based look-up table for measuring the electrical characteristics of a carbon-fiber electrode in pulse voltammetry. Using this instrument, the equivalent resistan...

journal_title：Journal of neuroscience methods

authors： Liao BY,Lio HP,Wang CY,Young MS,Ho MT,Lin MT

更新日期：1993-12-01 00:00:00

abstract：:Superpositioning of multiple 3-dimensional digital volumes is essential to extending neuroimaging capabilities from single-subject analysis to group evaluation. In this study, 6 rat heads were cryoplaned and digital images of the specimen blockface were organized into data volumes. The midline plane of each brain guid...

journal_title：Journal of neuroscience methods

authors： Santori EM,Toga AW

更新日期：1993-11-01 00:00:00

abstract：:Experimental recordings from neurons are generally difficult to quantify. Spike patterns and membrane potential cycles are often compared qualitatively and are hence limited by human resolution and judgement. This paper describes a statistical method for quantifying individual spike patterns and membrane potential flu...

journal_title：Journal of neuroscience methods

authors： Olsen OH,Murray-Smith D

更新日期：1993-10-01 00:00:00

abstract：:Currently there is no reliable retrograde tracing technique for visualization of dendritic morphologies of projection neurons. Here we describe a simple and efficient method that can be used to label neurons in Golgi-like fashion. The approach relies on activity-dependent uptake of tracer. For this purpose we inject t...

journal_title：Journal of neuroscience methods

authors： Jiang X,Johnson RR,Burkhalter A

更新日期：1993-10-01 00:00:00

abstract：:A method is described for the production of an electrode capable of monitoring and modulating the input-output relationship of thalamic neurons. Tungsten-in-glass electrodes were manufactured with the ability to simultaneously record lateral geniculate nucleus action potentials and associated, retinally evoked S-poten...

journal_title：Journal of neuroscience methods

authors： Godwin DW

更新日期：1993-09-01 00:00:00

abstract：:A hydraulically driven, digitally servo-controlled multi-axes rotary chair is described. This device generates motion profiles with the subjects head in the center of rotation mainly in order to adequately stimulate the semicircular canals which are sensitive sensors for angular accelerations. This newly developed app...

journal_title：Journal of neuroscience methods

authors： Probst T,Dabrowski H,Liebler G,Wist ER

更新日期：1993-08-01 00:00:00

abstract：:A mathematical model is presented by which the regeneration rate and initial delay for peripheral nerve regeneration can be calculated from sensory pinch test data following surgical repair of peripheral nerves. The model is based on the assumption that experimental variations in regeneration distances between animals...

journal_title：Journal of neuroscience methods

authors： Holmquist B,Kanje M,Kerns JM,Danielsen N

更新日期：1993-06-01 00:00:00

abstract：:We investigated the properties of biotinylated dextran-amine (BDA) as a neuroanatomical tracer at the electron microscopic level and we compared the results with those obtained previously with another tracer, the lectin Phaseolus vulgaris-leucoagglutinin (PHA-L). BDA was injected into various brain areas of rats. Foll...

journal_title：Journal of neuroscience methods

authors： Wouterlood FG,Jorritsma-Byham B

更新日期：1993-06-01 00:00:00

abstract：:We describe a novel procedure for combining immunocytochemistry with in situ hybridisation. In contrast to previously published procedures, the technique involves immunofluorescence followed by in situ hybridization and is particularly suitable for antigens which are labile or sensitive to in situ hybridization proces...

journal_title：Journal of neuroscience methods

authors： Priestley JV,Wotherspoon G,Savery D,Averill S,Rattray M

更新日期：1993-06-01 00:00:00

abstract：:Intracranial self-stimulation (ICSS) in the rat is a useful tool for studying the importance of various brain monoamines in positive reinforcement. The effects of compounds interacting with dopaminergic neurotransmission is measurable by studying the changes of reward thresholds. By computerisation of the analysis of ...

journal_title：Journal of neuroscience methods

authors： Kling-Petersen T,Svensson K

更新日期：1993-05-01 00:00:00

abstract：:The discovery of immediate early genes (IEG) has provided neuroscientists with a new functional mapping technique. Labelling of neural tissue for the protein product of IEG provides an activity map with single-cell resolution. When combined with labelling for the chemical identity of the neuron, this provides a powerf...

journal_title：Journal of neuroscience methods

authors： Smith DW,Day TA

更新日期：1993-04-01 00:00:00

abstract：:We describe a simple, scaled-down instrument which enables accurate, reproducible stereotaxic placements into specific sites in the brain of the newborn rat. The instrument is specially designed for the administration of long-term hypothermia, yet permits the use of alternative methods of anesthesia. The design of the...

journal_title：Journal of neuroscience methods

authors： Cunningham MG,McKay RD

更新日期：1993-04-01 00:00:00

abstract：:We used 24 sacral dorsal roots of the rat to analyze amplitude and area changes of biphasic and monophasic compound action potentials (CAPs) at 4 conduction distances. Both the CAPs of the A-fiber and C-fiber were analyzed. The changes were examined with the paired t test and linear regression. All the variables decre...

journal_title：Journal of neuroscience methods

authors： Jaw FS,Tsao HW,Yu HJ,Yen CT

更新日期：1993-03-01 00:00:00

abstract：:Background muscle activity (BGR) exerts a strong influence on stretch reflex amplitudes. While it is considered important to correct for this influence, which method best able to eliminate the effects of BGR remains unknown. We have therefore compared one previously described and one novel correction method which resp...

journal_title：Journal of neuroscience methods

authors： Bloem BR,van Dijk JG,Beckley DJ,Zwinderman AH,Remler MP,Roos RA

更新日期：1993-02-01 00:00:00

abstract：:We have developed and characterized a method for the rapid autoradiographic determination of receptor sites for the non-competitive NMDA receptor antagonist, MK-801, using an iodinated form of the compound, (+)-3-[125I]-iodo-MK-801. The binding site was shown to exhibit those criteria necessary for its definition as a...

journal_title：Journal of neuroscience methods

authors： Jacobson W,Cottrell GA

更新日期：1993-01-01 00:00:00

abstract：:A simple method is described for achieving a salt bridge within a patch pipette. The tip of the pipette is filled with a Cl(-)-free solution that bathes the membrane patch. Above this is a Cl(-)-containing solution that surrounds the recording Ag/AgCl wire electrode. Addition of 0.07% (w/v) agarose to the solution in ...

journal_title：Journal of neuroscience methods

authors： Kleene SJ

更新日期：1993-01-01 00:00:00

abstract：:A modification of a commercially available digital-audio tape (DAT) for DC recordings such as those used in patch clamp and other electrophysiological experiments is introduced. The modified DAT can record data from 2 channels plus a separate trigger signal for up to 2 h. The bandwidth ranges from direct current to 21...

journal_title：Journal of neuroscience methods

authors： Lüscher C,Frei P,de Limoges D

更新日期：1992-12-01 00:00:00

abstract：:We have attempted to demonstrate the technical requirements and performance of a microscopical assay using a densitometric application of image analysis to measure immunohistochemical stain intensity. Not surprisingly, the techniques required were more demanding than those used for the quantification of field and obje...

journal_title：Journal of neuroscience methods

authors： Cowen T,Thrasivoulou C

更新日期：1992-10-01 00:00:00

abstract：:Four different fluorochromes were injected into adjacent cervical spinal cord segments, 1 unique tracer per segment. Each tracer, Fluoro-Gold, Fast Blue, Diamidino Yellow dihydrochloride and Propidium Iodide, was taken up by axonal terminals and transported intra-axonally in a retrograde direction to the cell bodies. ...

journal_title：Journal of neuroscience methods

authors： Akintunde A,Buxton DF

更新日期：1992-10-01 00:00:00

abstract：:Here we describe a method for intracellularly injecting mixtures of the fluorescent dye Lucifer Yellow and the permanent tracers HRP or biocytin into aldehyde-fixed slices of the dorsal lateral geniculate nucleus in young postnatal cats. Lucifer Yellow was used for visual control in the injection procedure and the inc...

journal_title：Journal of neuroscience methods

authors： Coleman LA,Friedlander MJ

更新日期：1992-09-01 00:00:00

abstract：:An improved method using HRP for visualizing axons in whole-mount preparations is described. Application of this method to preparations of regenerating frog peripheral nerves maintained in organ culture was used to show that regeneration following nerve crush begins after about 3 days. ...

journal_title：Journal of neuroscience methods

authors： Tonge D,Edbladh M,Edström A,Kanje M

更新日期：1992-08-01 00:00:00